Thermo Scientific™ Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Supplier:  Thermo Scientific™ 32054

Catalog No. PI32054

Description

Thermo Scientific™ Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Highly diluted primary antibodies can be used with ABC Staining Kits, producing comparable stain intensity to other methods that require higher concentrations of antibody. Kits with a secondary antibody are selected by the species of primary antibody to be used. For example, if the primary antibody (IgG) is produced in mice, select the ABC Mouse IgG Kit. All you need to create a sensitive detection system is your specific primary antibody, an ABC Kit with biotinylated antibody and a substrate for horseradish peroxidase.The Standard Kits include only the avidin and biotinylated enzyme. They are useful if an ABC Kit is not available for your specific species of primary antibody, or if the primary antibody is directly labeled with a Thermo Scientific EZ-Link Biotinylation Reagent. The biotinylated antibody for your specific application can then be used, along with blocking serum and a Standard ABC Kit.

Highlights:

• Produce very low background staining
• Require lower primary antibody concentrations than standard methods
• Ideal for immunohistochemistry, ELISA, and Western blotting applications

ABC Kits

• Available with horseradish peroxidase
• Contain a biotinylated secondary antibody in a choice of species – the species should be selected based on the species of the primary antibody to be used

Standard Kits

• Available with a choice of alkaline phosphatase or horseradish peroxidase
• Suitable for use when an ABC kit is not available for the species of the primary antibody or if the specificity of the antibody in the kit is not appropriate for the application
• Do not include secondary antibody or blocking serum – secondary antibody and blocking serum appropriate to the application should be purchased separately

Ultrasensitive ABC Kits

• Available with horseradish peroxidase
• Provide greater sensitivity than ABC Kits without increased background staining
• Supply the extra sensitivity needed for localizing antigens present in very small quantities
• Permit approximately five-fold higher dilutions of primary antibody to be used than do ABC Kits

Includes:

Biotinylated affinity-purified secondary antibody, 1mL (except Standard Kits); Appropriate blocking serum, 3mL (except Standard Kits); Avidin reagent, 2mL; Biotinylated enzyme reagent, 2mL

Specifications

• Description: Pierce Ultrasensitive ABC Peroxidase Rabbit IgG Staining Kit
• Quantity: At least 10 mL of ABC staining solution
• Substrate Type: HRP (Horseradish Peroxidase) Substrate
• For Use With (Application): Immunodetection, IHC (Immunohistochemistry)

• Detection Method: Colorimetric, Biotin-Based, Chromogenic
• Content And Storage: Sufficient For: At least 10 mL of ABC staining solution
• Reagent A (Avidin), 2 mL
• Reagent B (Biotinylated HRP), 2 mL
• Blocking Serum (goat), 3 mL
• Biotinylated Goat Anti-Rabbit IgG, 1 mL
• Mixing bottles with dropper dispenser tips, 3
  
  Store all reagents at 4°C
• Product Line: Pierce™
• Product Type: Staining Kit

For Research Use Only. Not for use in diagnostic procedures.