Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular, Thermo Scientific Chemicals

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Catalog Number	Mfr. No.	Quantity	Packaging
AC327111000	Thermo Scientific Chemicals 327111000	100 mL	Glass bottle
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AC327115000	Thermo Scientific Chemicals 327115000	500 mL	Glass bottle
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AC327110025	Thermo Scientific Chemicals 327110025	2.5 L	Glass bottle
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Description

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

• Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
• When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
• It is also used to generate DNA minisatellite fingerprints

Specifications

• Chemical Name or Material: Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0
• Name Note: DNase, RNase and Protease Free
• Molecular Formula: C₆H₆O
• UN Number: 2927
• Merck Index: 14, 7241
• Grade: Molecular Biology
• Specific Gravity: 1.28
• pH: 7.8 to 8.2 (25°C)

• Color: Yellow to Orange
• CAS: 136112-00-0, 67-66-3, 123-51-3
• MDL Number: MFCD00133763
• Density: 1.2800g/mL
• Formula Weight: 94.11
• Packaging: Glass bottle
• Treatment(s): Stabilized
• Physical Form: Solution

Safety and Handling

Suspected of causing genetic defects, Suspected of damaging the unborn child, Causes damage to organs through prolonged or repeated exposure, Causes severe skin burns and eye damage, May cause drowsiness or dizziness, Suspected of causing cancer, Repeated exposure may cause skin dryness or cracking, Toxic if swallowed, in contact with skin or if inhaled

Germ cell mutagenicity (category 2), Reproductive toxicity (category 2), Specific target organ toxicity after repeated exposure (category 1), Skin corrosion/irritation (category 1), Specific target organ toxicity after single exposure (category 3), Carcinogenicity (category 2), Acute toxicity (category 3)

RUO – Research Use Only