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Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Supplier: Thermo Scientific™ 23030
Description
Thermo Scientific™ Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.
Highlights:
- Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
- Reaction can be monitored by measuring the decrease in absorbance at 300nm
Specifications
Thiol | |
Unreactive Group | |
Pierce™ | |
Medium (10 to 30 Å) | |
25 g | |
NEM |
Upon receipt store at -20°C. | |
Blocking Groups | |
Maleimide | |
Solid | |
Water |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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