Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

Supplier:  Gibco™ A14697

Catalog No. A14697

Description

• Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
• Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
• WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
• TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
• Neomycin resistance gene for selection of stable cell lines with Geneticin™
• pUC origin for high copy replication and maintenance of the plasmid in E. coli

Specifications

• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Plasmid: High Copy Plasmid
• Product Type: TA Cloning Kit
• Protein Tag: Untagged
• Replication Origin: pUC Origin
• Selection Marker Eukaryotic: NeoR
• Vector: pcDNA

• Cloning Method: TOPO™-TA
• Content And Storage: Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
  Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.
• For Use With (Application): Protein Production, Constitutive Expression
• Product Line: TOPO™
• Promoter: CMV
• Quantity: 20 μg
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Selection Marker Promoter: SV40 Promoter

For Research Use Only. Not for use in diagnostic procedures.