Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Supplier: Thermo Scientific™ 26617
Thermo Scientific™ PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.
Size range – 10 proteins spanning 10 to 180kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands – color-coded bands of similar intensity for easy visualization
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Two reference bands – orange at 70kDa and green at 10kDa
Membrane-compatible – colored bands transfer to membranes for Western blotting
Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots
|Contents: 10 vials of 250 µL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C
|3 colors: Blue, Orange, Green|
|180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa|
|10 x 250 μL|
|Western Blotting, SDS-PAGE|
|Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)|
|10 to 180 kDa|
|Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels|
|Approved for shipment on Wet or Dry Ice|
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