Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier:  Thermo Scientific™ 26617

Catalog No. PI26617

Description

Thermo Scientific™ PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Highlights:

• Size range – 10 proteins spanning 10 to 180kDa

• Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

• Sharp bands – color-coded bands of similar intensity for easy visualization

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

• Two reference bands – orange at 70kDa and green at 10kDa

• Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN₃ and 33% glycerol.

Recommended for:

Monitoring protein migration during SDS-polyacrylamide gel electrophoresis; Monitoring protein transfer onto membranes after Western blotting; Sizing of proteins on SDS-polyacrylamide gels and Western blots

Specifications

• Content And Storage: Contents: 10 vials of 250 µL each
  
  Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol
  
  Storage: Upon receipt store at -20°C
  
  
• Number of Markers: 10
• Ready to Load: Yes
• Stain Type: 3 colors: Blue, Orange, Green
• Molecular Weight (g/mol): 180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
• Quantity: 10 x 250 μL
• System Type: Western Blotting, SDS-PAGE

• Detection Method: Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
• Product Type Specs: Protein Ladder
• Size Range: 10 to 180 kDa
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Product Line: PageRuler™
• Shipping Condition: Approved for shipment on Wet or Dry Ice