p300 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA1848

Catalog No. PA1848

Description

PA1-848 detects p300 from human cells. PA1-848 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ∽265 kDa protein representing p300 from HeLa cell lysate. The PA1-848 immunogen is a synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300. The PA1-848 immunizing peptide (Cat. # PEP-053) is available for use in neutralization and control experiments.

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Specifications

• Antigen: p300
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EP300
• Gene Alias: A430090G16; A730011L11; E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; Ep300; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; KAT3B; P300; p300 HAT; Protein propionyltransferase p300; RSTS2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2033
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, Immunocytochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q09472
• Gene Symbols: EP300
• Immunogen: Synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG