Invitrogen™ NuPAGE™ 12%, Bis-Tris, 1.0 mm, Mini Protein Gels

NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions.

Catalog Number	Mfr. No.	Wells	Quantity
NP0341BOX	Invitrogen™ NP0341BOX	10-well	10 Gels/Box
NP0341PK2	Invitrogen™ NP0341PK2	10-well	2 Gels/Box
NP0342BOX	Invitrogen™ NP0342BOX	12-well	10 Gels/Box
NP0342PK2	Invitrogen™ NP0342PK2	12-well	2 Gels/Box
NP0343BOX	Invitrogen™ NP0343BOX	15-well	10 Gels/Box
NP0344BOX	Invitrogen™ NP0344BOX	1-well	10 Gels/Box
NP0346BOX	Invitrogen™ NP0346BOX	2D-well	10 Gels/Box
NP0349BOX	Invitrogen™ NP0349BOX	17-well	10 Gels/Box

Description

NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE™ Novex™ Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB210010).

Specifications

• For Use With (Equipment): Mini Gel Tank, XCell SureLock Mini-Cell
• Gel Size: Mini
• Gel Type: Bis-Tris
• Mode of Separation: Molecular Weight
• Shipping Condition: Room Temperature
• Length (Metric): 8 cm

• Gel Percentage: 12%
• Gel Thickness: 1.0 mm
• Separation Type: Denaturing
• Product Line: NuPAGE™
• Storage Requirements: Store at 4–25°C. Do not freeze.
• Width (Metric): 8 cm

Safety and Handling

ShelfLife : 12 Months