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MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium-15N
Provides reagents to make a chemically defined autoinducing liquid culture medium used to produce [U-15N] labeled proteins for NMR spectroscopy. Novagen™ Overnight Express™ Autoinduction NMR Medium- 15N system includes four concentrated sterile solutions.
Supplier: MilliporeSigma™ 717593
Description
- Autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density
- Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
- Includes four concentrated sterile solutions:
- OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
- OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
- OnEx Solution 3 provides magnesium for maximal cell density
- OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels
Specifications
| 1 L Kit | |
| Multiple storage requirements. Ok to freeze. | |
| NMR Medium-15N |
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