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Invitrogen™ Neon™ Transfection System 100 μL Kit

Neon™ Transfection System 100 μL Kit

Supplier:  Invitrogen™ MPK10096

Encompass_Preferred

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Catalog No. MPK10096


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Includes:

30mL Resuspension Buffer R, 30mL Resuspension Buffer T, 2 x 150mL Electrolytic Buffer E2, 96 x 100μL Neon™ Tips, 20 Neon Electroporation Tubes


Description

Description

  • For use with the Neon Transfection System
  • Use this kit for transfection volumes of 100μL, containing 5 x 105-2 x 106 adherent cells or 1 x 106-5 x 106 suspension cells
  • Cells that have been transfected using the included 100μL Neon Tips are then ready to be washed and plated into a 6-well, 60mm, or 10cm culture dish, depending on protocol

Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Noncoding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Stem Cells, Primary Cells, Hard-to-Transfect Cells
Plasmid DNA, Synthetic siRNA
5 x 10^5 - 2 x 10^6 cells/mL (Adherent Cells), 1 x 10^6 - 5 x 10^6 cells/mL
Transfection
Neon™
96 x 2 Reactions
Room Temperature
Neon™ Transfection System
• 30 mL Resuspension Buffer R
• 30 mL Resuspension Buffer T
• 2 x 150 mL Electrolytic Buffer E2
• 96 x 100 μL Neon™ Tips
• 20 Neon™ Electroporation Tubes

Store the buffer box 4°C and the tips & tubes box at room temperature.
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Electroporation
Neon™ Transfection System
96 x 2
Transfection System Kit
Yes
2x10^4 - 6 x10^8 cells; Varies by cell type
100μl
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For Research Use Only. Not for use in diagnostic procedures.