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Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells
Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.
Supplier: Invitrogen™ 00450051
Description
- Mouse splenocytes were stimulated with ConA (3μg/mL) for two days, followed by recombinant IL-2 (20ng/mL) and IL-4 (20ng/mL) for three days
- The cells were then restimulated with immobilized anti-mouse CD3 (10μg/mL) and soluble anti-mouse CD28 (2μg/mL) in the presence of Brefeldin A for 5 hours
- These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ
- The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at −80°C for up to six months or at 2 to 8°C for up to one month
Specifications
| Mouse Cytokine Positive Control Cells | |
| eBioscience™ | |
| Flow Cytometer |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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