Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody, Invitrogen™

Mouse Monoclonal Secondary Antibody

Supplier:  Thermo Scientific MA181864

Catalog No. MA181864

Description

This antibody recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human and Cynomolgus monkey IgG. For Flow cytometry, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG Fc (CH2 domain)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Immunogen: Human IgG polyclonal.
• Quantity: 200 μg
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Clone: 8A4
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Form: Liquid