Edvotek™ Molecular Weight Determination of Proteins

Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis.

Supplier:  Edvotek™ 110

Catalog No. S11110

Includes: instructions, prestained LyphoProtein™ samples, gel loading solution, agarose, electrophoresis buffer, stain, SDS solution

Description

Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and ''unknowns'' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is EDVOTEK™´s formulation of protein grade agarose, which provides an alternative to the use of polyacrylamide gels.

Specifications

• For Use With (Application): Determine protein subunit molecular weights using horizontal electrophoresis
• Requires: Horizontal electrophoresis apparatus, power supply, micropipette with tips, microwave oven or hot plate, 250 mL or 1 L flask, larger beaker, lab tape or markers, hot gloves, safety goggles and lab gloves, and distilled or deionized water. For Staining with Protein InstaStain™ (optional): methanol, glacial acetic acid, small plastic tray or large weigh boat, plastic wrap, white light box (recommended), rocking platform, and pipet pump or bulb
• Content And Storage: Some components require freezer storage.

• Includes: Instructions, pre-stained LyphoProtein™ gel marker, pre-stained LyphoProtein™ samples, practice gel loading solution, Protein Agarose™ powder, Tris-Glycine-SDS powdered buffer, Protein InstaStain™, transfer pipets, and 1 mL pipet
• Time Range: Gel Prep: 40 minutes; Electrophoresis: 60 minutes
• Class Size: 6 Lab Groups