Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)

Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters

Supplier:  Invitrogen™ R37601

Catalog No. R37601

Includes: Component A: 10 x 1mL vials; Component B: 1 x 10 vials, dried down

Description

• Allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability: intracellular esterase activity and plasma membrane integrity
• Based on cell-permeable dye for staining of live cells and cell-impermeable dye for staining of dead and dying cells
• Adapted for imaging platforms by optimizing for common green and red imaging filters used with FITC and Texas Red
• Live cell component produces intense, uniform green fluorescence in live cells (ex/em 488 nm/515nm
• Dead cell component produces predominantly nuclear red fluorescence (ex/em 570nm/602nm) in cells with compromised cell membranes, strong indicator of cell death and cytotoxicity
• Sensitive detection of key measures of cell health: viability, live/dead ratio, and cytotoxicity
• Ease of use with minimal dilutions
• Live cells are distinguished by presence of ubiquitous intracellular esterase activity as determined by the enzymatic conversion of virtually nonfluorescent cell-permeant calcein AM to intensely fluorescent calcein, which is well-retained within live cells
• Red component is cell-impermeant and therefore only enters cells with damaged membranes
• In dying and dead cells bright red fluorescence is generated upon binding to DNA
• Background fluorescence levels are inherently low with this assay technique because dyes are virtually nonfluorescent before interacting with cells

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Dry Ice

Specifications

• Cell Type: Mammalian Cells
• Content And Storage: • Component A: 10 x 1 mL vials
  • Component B: 1 x 10 vials (dried down)
  
  Store at -20°C
  
• Detection Method: Fluorescence
• For Use With (Equipment): Floid Cell Imaging System, Fluorescence Microscope
• Dye Type: Other Label(s) or Dye(s)
• Excitation Wavelength Range: 488, 570 nm
• Format: Tube(s)
• Product Line: LIVE/DEAD™, Molecular Probes™
• Shipping Condition: Dry Ice

• Color: Red, Green
• Description: LIVE/DEAD™ Cell Imaging Kit (488/570)
• For Use With (Application): Viability Assay
• Product Type: Cell Imaging Kit
• Emission: 515, 602 nm
• Form: Frozen
• Incubation Time: 15 min
• Quantity: 1 mL