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Invitrogen™ ImaGene Red™ C12RG lacZ Gene Expression Kit
Substrates cleaved within cell for fluorescent product
Supplier: Invitrogen™ I2906
Description
The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays
Order Info
Shipping Condition: Room Temperature
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| β-Gal (lacZ) Assay | |
| Room Temperature | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| DetectaGene |
| Fluorescent | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| C12RG | |
| Chemical Substrate | |
| Kit |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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