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Invitrogen™ Image-iT™ Fixation/Permeabilization Kit

Convenient-to-use fixation/permeabilization kit that contains all necessary reagents to prepare cells for antibody staining and imaging

Supplier:  Invitrogen™ R37602


Catalog No. R37602

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Includes: All components are provided at 1X and are ready to use. Kit Contains: High purity 4% formaldehyde in PBS, pH 7.3; 0.5% Triton X-100; 3% BSA, fraction V, delipidated, New Zealand source, in DPBS; PBS, pH 7.4



  • Components help preserve cell morphology and reduce background staining and come in practical box with single-use vials and easy-to-follow protocols
  • Complete fixation/permeabilization kit with ready-to-use components and room temperature storage
  • Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications
  • Convenient storage box, 1X ready-to-use components
  • Compatible with analysis of most cellular antigens
  • Reduced background staining
  • Components were chosen to meet wide range of sample preparation requirements demanded for successful fluorescent imaging of fixed and permeabilized cell
  • Reagents optimaized for both preservation of 3D cellular structure and adequate antibody access to intracellular antigenic sites
  • Kit components were extensively tested with both nuclear and cytoplasmic targets and showed equal or superior results compared to standard reagents
  • Cells are treated sequentially with fixative, permeabilization solution, and blocking buffer according to simplified and easy-to-follow protocol: This procedure affords optimal access to intracellular structures for antibodies and fixed cell dyes and maintains cellular morphology for subsequent fluorescent cell imaging
  • Decreased background staining, consequently improving signal-to-noise levels and image quality

Cell Analysis, Cellular Imaging, IHC Staining and Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Sample Preparation, Immunohistochemistry (IHC)

Order Info

Shipping Condition: Room temperature



Fixation/Permeabilization Kit
Fixation Reagent, Permeabilization Reagent, Triton X-100
Room Temperature
Image-iT™, Molecular Probes™
Fixative Solution: 20 mL
  • Permeabilization solution: 20 mL
  • Blocking solution: 12 x 10 mL
  • Wash Buffer: 500 mL
  • Store at ≤25 °C.
  • SDS


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