Invitrogen™ Image-iT™ Fixation/Permeabilization Kit

Convenient-to-use fixation/permeabilization kit that contains all necessary reagents to prepare cells for antibody staining and imaging

Supplier:  Invitrogen™ R37602

Catalog No. R37602

Includes: All components are provided at 1X and are ready to use. Kit Contains: High purity 4% formaldehyde in PBS, pH 7.3; 0.5% Triton X-100; 3% BSA, fraction V, delipidated, New Zealand source, in DPBS; PBS, pH 7.4

Description

• Components help preserve cell morphology and reduce background staining and come in practical box with single-use vials and easy-to-follow protocols
• Complete fixation/permeabilization kit with ready-to-use components and room temperature storage
• Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications
• Convenient storage box, 1X ready-to-use components
• Compatible with analysis of most cellular antigens
• Reduced background staining
• Components were chosen to meet wide range of sample preparation requirements demanded for successful fluorescent imaging of fixed and permeabilized cell
• Reagents optimaized for both preservation of 3D cellular structure and adequate antibody access to intracellular antigenic sites
• Kit components were extensively tested with both nuclear and cytoplasmic targets and showed equal or superior results compared to standard reagents
• Cells are treated sequentially with fixative, permeabilization solution, and blocking buffer according to simplified and easy-to-follow protocol: This procedure affords optimal access to intracellular structures for antibodies and fixed cell dyes and maintains cellular morphology for subsequent fluorescent cell imaging
• Decreased background staining, consequently improving signal-to-noise levels and image quality

Cell Analysis, Cellular Imaging, IHC Staining and Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Sample Preparation, Immunohistochemistry (IHC)

Order Info

Shipping Condition: Room temperature

Specifications

• Type: Fixation/Permeabilization Kit
• Reagent Type: Fixation Reagent, Permeabilization Reagent, Triton X-100
• Shipping Condition: Room Temperature

• Product Line: Image-iT™, Molecular Probes™
• Content And Storage: Fixative Solution: 20 mL
• Permeabilization solution: 20 mL
• Blocking solution: 12 x 10 mL
• Wash Buffer: 500 mL
• Store at ≤25 °C.