Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor™ 488
GREENER_CHOICE

Detects lipid peroxidation in cells

Supplier:  Invitrogen™ C10446

Encompass_Preferred

Catalog No. C10446

  • $597.65 / Each of 1

    Save 4%

    Reg : $622.00

    The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
    Learn More
Add to Cart

Includes: Sufficient for five 96-well plates or 100 coverslips. Includes positive control compound.

Description

Description

Kit leverages copper-catalyzed click chemistry and linoleamide alkyne (LAA) reagent (alkyne-modified linoleic acid) to detect lipid peroxidation-derived protein modifications in fixed cells. Linoleic acid is most abundant polyunsaturated fatty acid found in mammals and its lipid peroxidation products likely account for majority of lipid-derived protein carbonyls.

  • Optimized for detection of lipid peroxidation in cells using click chemistry and Alexa Fluor™ 488 azide
  • Multiplexible and compatible with traditional fluorescence microscopy, high content screening (HCS), and flow cytometry
  • Provided as a complete kit sufficient for five 96-well plates or 100 coverslips
  • Form: Frozen
  • Excitation Class: Visible

How It Works

  • Click-iT Lipid Peroxidation Imaging Kit contains cumene hydroperoxide as ositive control for induction of lipid peroxidation and all of components for click reaction, including LAA, and Alexa Fluor™ 488 azide
  • LAA can be incubated with cells, where it incorporates into cellular membranes
  • Upon lipid peroxidation, LAA is oxidized and produces 9- and 13- hydroperoxy-octadecadienoic acid (HPODE)
  • Hydroperoxides decompose to multiple α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains
  • Alkyne-containing modified proteins can be subsequently detected using Click-iT chemistry and multiplexed with other probes appropriate for fixed cells

  • Click-iT™ LAA (linoleamide alkyne) is stand-alone reagent provided as five pack set of single-use vials for maximum flexibility in assays for lipid peroxidation
  • Reagent may be combined with variety of azide-modified detection reagents and other reagents

Cell Analysis, Cell Health and Oxidative Stress with Click Chemistry, Cell Viability, Proliferation and Function, Cellular Imaging, Click Chemistries, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Labeling Chemistry, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Red, Green
Frozen
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.