Invitrogen™ CellLight™ Plasma Membrane-RFP, BacMam 2.0

Provides easy way to label the plasma membrane with red fluorescent protein in live cells

Supplier:  Invitrogen™ C10608

Catalog No. C10608

Description

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the myristolyation/palmitoylation sequence from Lck tyrosine kinase. You can observe plasma membrane-RFP behavior in live cells without staining internal membranes, and also use multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

BacMam Technology

CellLight Plasma Membrane-RFP, BacMam 2.0, is a fusion construct of the myristolyation/palmitoylation sequence from Lck tyrosine kinase and TagRFP, providing accurate and specific targeting to cellular plasma membrane-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

• Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
• Highly efficient: up to 90% transduction of wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying dose
• Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

Cell Analysis, Cell Structure, Cellular Imaging, Immunocytochemistry (ICC), Immunofluorescence (IF), Plasma Membrane

Order Info

Shipping Condition: Wet Ice

Specifications

• Color: Red-Orange, Orange
• Excitation Wavelength Range: 555⁄584
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope
• Detection Method: Fluorescent
• Form: Liquid
• Shipping Condition: Wet Ice
• Label Type: Fluorescent Dye
• SubCellular Localization: Plasma Membrane

• Content And Storage: Store at 2°C to 6°C, protected from light. Do Not Freeze.
• Dye Type: RFP (TagRFP)
• Quantity: 1 vial
• Emission: Visible
• Product Line: CellLight™
• Technique: Fluorescence Intensity
• Product Type: Plasma Membrane Stain

For Research Use Only. Not for use in diagnostic procedures.