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Invitrogen™ APF (Hydroxyl Radical, Hypochlorite or Peroxynitrite Sensor)

Used together, these two ROS indicators can selectively detect hypochlorite anion

Supplier:  Invitrogen™ A36003

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Catalog No. A36003


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Description

Description

Aminophenyl fluorescein (APF) and hydroxyphenyl fluorescein (HPF, H36004), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H2DCFDA, D399).

  • APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation
  • Both of these new fluorescein derivatives are nonfluorescent until they react with hydroxyl radical or peroxynitrite anion
  • APF will also react with hypochlorite anion
  • Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima approx. 490/515nm), making them compatible with any instrument that can detect fluorescein

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative Stress, Reactive Oxygen Species

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