Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes

The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil.

Supplier:  Applied Biosystems™ A42358

Encompass_Preferred

Catalog No. A42358


Add to Cart

Description

Description

Stool and soil are often the sample types of choice for scientists in the microbiome field. However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now. The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.

Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in ∼60 minutes
• Compatible with stool, soil, and swabs contained in transport medium
• Mechanical disruption via bead beating in 96-well plate or individual tubes
• Elution volumes ranging from 50 to 200 μL

Maximize sample input and nucleic acid recovery in minimal time
Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate. When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ∼60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ∼50 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 100 μL of elution buffer.

Two bead beating formats available
High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents. If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing. Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with stool samples that are commonly used for microbiome research.

The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50-100 μL of elution buffer for use in downstream applications.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 80 mL Lysis Buffer; room temperature
• 50 mL Binding Solution; room temperature
• 200 mL Wash Buffer; room temperature
• 20 mL Elution Solution; room temperature
• 4 mL Proteinase K; room temperature
• 2 mL Total Nucleic Acid Binding Beads; room temperature
• 100 Tubes; room temperature

Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
RNA and DNA
Not High-throughput Compatible (Manual)
Room Temperature
Bacteria: ≤500 μL
Biofluids: ≤500 μL
Fecal: ≤100 mg (200 μL for samples in storage solution)
Soil: ≤250 mg
Yeast: ≤500 μL
Magnetic Bead
50 to 200 μL
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
100 Preps
60 min.
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.