Invitrogen™ LyticBLAzer™-FRET B/G Homogeneous Kit

LyticBLAzer™ -FRET B/G Substrate Kits provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates.

Catalog Number	Mfr. No.	Quantity
K1148	Invitrogen™ K1148	150 mL
K1149	Invitrogen™ K1149	3.75 L

Description

The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ FRET B/G Substrate Kit Advantages:

• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times

LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times

Specifications

• Product Line: LyticBLAzer™
• Content And Storage: LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be desiccated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22°C to 25°C, protected from direct light.
  
  The 150 mL kit is sufficient for 3,750 x 40 μL standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 μL standard protocol well assays. Bulk quantities of loading kits are available upon request.
  
  LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20C, desiccated
• Product Type: Kits for Analysis of Beta-Lactamase Activity

• For Use With (Equipment): Fluorometer, Microplate Reader
• Detection Method: Fluorescent

For Research Use Only. Not for use in diagnostic procedures.