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LL/2 (LLC1) (mouse Lewis lung carcinoma) Whole cell lysate, Non-denatured; Abnova

Non-denatured whole cell lysate of LL/2 (LLC1) (mouse Lewis lung carcinoma)

Supplier:  Abnova Corporation L059V2

Catalog No. 89-014-398


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Description

Description

  • Tissue: Lung
  • Host: Mouse
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

Specifications

Lung
Whole cell lysate (non-denatured)
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
200 μg
Mouse
Immunoprecipitation, Western Blot
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
12.5% SDS-PAGE Stained with Coomassie Blue.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Recommendations

SDS
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