Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

Determines the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation.

Supplier:  Invitrogen™ L34975

Catalog No. 50-112-1531

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a red laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Optimal brightness

• The LIVE/DEAD™ Fixable Near-IR stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser
• The near-IR fluorescent reactive dye has an excitation maximum of ∼633 nm making it ideal for use with the red or HeNe laser with an emission of ∼750 nm
• Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments

Specifications

• Cell Permeability: Impermeant
• Color: Near-IR
• Description: LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation
• For Use With (Application): Viability Assay
• Product Type: Stain
• Emission: 775 nm
• Form: Solid
• Product Line: LIVE/DEAD™
• Shipping Condition: Room Temperature

• Cell Type: Eukaryotic Cells
• Content And Storage: Contains 5 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.
  
  Store at -20°C.
  
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometer
• Dye Type: LIVE/DEAD™ FixableNear-IR Dead Cell Stain
• Excitation Wavelength Range: 633 nm
• Format: Tube(s)
• Quantity: 80 assays
• Solubility: DMSO (Dimethylsulfoxide)

For Research Use Only. Not for use in diagnostic procedures.