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Edvotek™ Isolation of E. Coli Chromosomal DNA
Isolate chromosomal DNA from E. Coli.
Supplier: Edvotek™ 203
Includes: instructions, Chromosomal LyphoCells™, various solutions and buffers, agarose powder, FlashBlue™ Stain.
Requires :
- Horizontal gel electrophoresis apparatus
- DC power supply
- Automatic micropipet with tips
- Waterbath
- Visualization system (white light)
- 50 and 250mL beakers or flasks
- 5 and 10mL pipets
- Test tubes for DNA resuspension
- 99–100% Isopropanol (70% Isopropyl rubbing alcohol can be substituted)
- Distilled or deionized water
Description
Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.Specifications
| Isolating chromosomal DNA | |
| 20 DNA Isolations and 5 Gels | |
| 1 hour 45 minutes |
| Instructions, Chromosomal LyphoCells™, Various solutions and buffers, Agarose powder, FlashBlue™ stain | |
| Water bath, pipet pumps or bulbs, lab glassware, distilled or deionized water, 95-100% isopropanol. For Optional Electrophoresis: Electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, misc. labware, white light visualization system, and photodocumentation system. | |
| Room temperature |
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