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  7. Isoamyl Alcohol, MP Biomedicals™

Isoamyl Alcohol, MP Biomedicals™

Catalog Number MP215507890
Click to view available options
Quantity:
100 mL
500 mL
1 L
123-51-3
C5H12O
88.15
PHTQWCKDNZKARW-UHFFFAOYSA-N
3-methyl-1-butanol, isoamyl alcohol, isopentyl alcohol, isopentanol, 3-methylbutanol, 1-butanol, 3-methyl, isoamylol, isobutylcarbinol, iso-amylalkohol, isobutyl carbinol
31260
CHEBI:15837
3-methylbutan-1-ol
CC(C)CCO
123-51-3
C5H12O
88.15
PHTQWCKDNZKARW-UHFFFAOYSA-N
3-methyl-1-butanol, isoamyl alcohol, isopentyl alcohol, isopentanol, 3-methylbutanol, 1-butanol, 3-methyl, isoamylol, isobutylcarbinol, iso-amylalkohol, isobutyl carbinol
31260
CHEBI:15837
3-methylbutan-1-ol
CC(C)CCO

Description

Routinely used in molecular biology. Isoamyl Alcohol, MP Biomedicals is one of several isomers of amyl alcohol. It is especially utilized as a reagent in the purification of DNA, the extraction of ethidium bromide from DNA solutions and various other misc

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue.

  • Widely used in conjunction with phenol and chloroform (25:24:1—phenol:chloroform:isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction
  • Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions
  • It will also help in reducing the foaming during the extraction process

Isoamy alcohol has various miscellaneous uses, including:

  • HPLC analysis of various pharmaceuticals and metabolites
  • Analysis of oxidized and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria
  • Isolation of mRNA from a thermophilic cyanobacterium
  • Solvent for fats, resins and alkaloids
  • Manufacturing of isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers and smokeless powders
  • For dehydrating celloidin solutions
  • For determining fat in milk
Melting Point -117.2°C (literature)
Density 0.81g/mL (water=1) at 25°C (Lit.)
Boiling Point 131°C (literature)
Refractive Index n20/D 1.406 (Lit.)
Quantity 500 mL
Solubility Information Miscible with alcohol, ether, benzene, chloroform, petroleum ether, glacial acetic acid, oils.
Vapor Density 3.04 (air=1) (Lit.)
Vapor Pressure 2.4mmHg at 20°C, 2.37mmHg at 25°C (Lit.)
Formula Weight 88.15
Percent Purity ≥99%
Physical Form Liquid
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Safety and Handling

Recommended Storage Room Temperature (15–30°C).
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