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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli
Designed for cloning PCR products directly from a PCR reaction in just 5 minutes
$687.65 - $1306.00
Specifications
Bacterial or Yeast Strain | TOP10F´ |
---|---|
Cell Type | Chemically Competent |
Cloning Method | TOPO™-TA |
Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
Format | Kit |
Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
Description
Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Cloning, PCR Cloning
Specifications
TOP10F´ | |
TOPO™-TA | |
Kit | |
Cloning Kit | |
TOPO-TA Cloning Vectors |
Chemically Competent | |
Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
|
One Shot™ | |
T7, SP6 |
For Research Use Only. Not for use in diagnostic procedures.
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