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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells
For fast, efficient cloning and subsequent in vitro transcription/translation
Supplier: Invitrogen™ K460001
Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid
Description
By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and SP6 promoters for efficient in vitro transcription
- M13 forward and reverse primer sites for sequencing
- 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
- Kanamycin and ampicillin resistance for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
- Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies
Applications:
Cloning, PCR Cloning
Specifications
| TOP10 | |
| TOPO™-TA | |
| Kit | |
| Cloning Kit | |
| 25 Reactions |
| Chemically Competent | |
| Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
|
| One Shot™ | |
| T7, SP6 | |
| TOPO-TA Cloning Vectors |
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For Research Use Only. Not for use in diagnostic procedures.
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