Invitrogen™ T4 DNA Polymerase

DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity

Includes: T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165mM Tris acetate (pH 7.9), 330mM sodium acetate, 50mM magnesium acetate, 5mM DTT].

Catalog Number	Mfr. No.	Quantity
18-005-017	Invitrogen™ 18005017	50 U
18-005-025	Invitrogen™ 18005025	250 U

Description

• Applications: Labeling double-stranded linear DNA by replacement synthesis, Oligonucleotide-directed, site-specific mutagenesis, 3' end-labeling of double-stranded DNA, Polishing both 5' or 3 overhangs to make blunt ends
• Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid
• Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested
• Unit Definition: One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template primer
• Unit Reaction Conditions: 50mM glycine-NaOH (pH 8.8), 16.6mM (NH₄)₂SO₄, 6mM MgCl₂, 6.5μM EDTA, 10mM 2-mercaptoethanol, 0.165mg/mL BSA, 1.6mg/mL DNase I-nicked salmon testes DNA, 0.33mM dCTP, 0.33mM dATP, 0.33mM dGTP, 0.33mM dTTP, 76nM [^{3H]dTTP, and enzyme in 0.1mL for 30 min. at 37°C}
• A T4 DNA Polymerase Technical Bulletin is available

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.
• Polymerase: T4 DNA Polymerase
• Shipping Condition: Wet Ice

• Description: DNA Polymerase
• Exonuclease Activity: 3' - 5'
• Hot Start: No

For Research Use Only. Not for use in diagnostic procedures.