Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Supplier:  Invitrogen™ 11754250

Catalog No. 11-754-250

Includes: 5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein

Description

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • 5X VILO Reaction Mix, 1,000 μL
  • 10X SuperScript III Enzyme Blend, 500 μL
  
  Store at –5 to –30°C.
  
  

• Format: Kit
• Reaction Speed: 60 min.
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Reduced
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 250 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Detection Method: qPCR
• GC-Rich PCR Performance: High
• Technique: Reverse Transcription
• Reverse Transcriptase: SuperScript III
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Master Mix
• Size (Final Product): Up to 10 kb

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.