Invitrogen™ PureLink™ Genomic DNA Mini Kit

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

Supplier:  Invitrogen™ K182002

Catalog No. K182002

Description

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no detectable contamination from carryover of protein or guanidine.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
  • 45 mL PureLink Genomic Digestion Buffer; room temperature
  • 50 mL PureLinkGenomic Wash Buffer 1; room temperature
  • 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
  • 50 mL PureLink Genomic Elution Buffer; room temperature
  • 5 mL RNase A (20 mg/mL); room temperature
  • 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
  • 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
  • 5 x 100 PureLink Collection Tubes; room temperature
  
  
• Sample Type: Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
• Elution Volume: 25 to 200 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 250 Preps
• Test Time: Up to 15 min. after cell lysis
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Cells: ≤10⁶
  Tissue: ≤25 mg
  Mouse Tail: 1 cm
  Rat Tail: 0.5 cm
  Whole Blood: ≤1 mL
  Yeast: ≤10⁷ cells
  FFPE: ≤8 sections of 5-15 μM thickness
  Oragene™ Preserved Saliva: ≤4 mL
  Blood Spots: ≤5 punches

• Isolation Technology: Silica Spin Column
• Scale: Mini
• Final Product Type: gDNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: ≤40 μg

For Research Use Only. Not for use in diagnostic procedures.