Invitrogen™ IL-6 Human ProQuantum Immunoassay Kit

Measure cytokine proteins with small sample consumption and high sensitvity on a qPCR instrument

Supplier:  Invitrogen™ A355735

Catalog No. A355735

Description

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury,and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts,activated T cells,activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells,T cells,B cells,and hepatocytes. In addition,IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation,IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor,alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Accession Number: P05231
• Assay Sensitivity: <0.05pg/mL
• Product Type: ProQuantum
• Target Species: Human
• For Use With (Equipment): qPCR Instruments
• Gene Symbol: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
• Label or Dye: FAM™
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Test Time: 30 min.

• Assay Range: 0.064 to 10000 pg/mL
• Detection Method: Taqman™ Real-time PCR
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Cytokine protein quantitation
• Gene ID (Entrez): 3569
• Kit Contents: Assay Dilution Buffer<Br>Antibody-Conjugate A<Br>Antibody-Conjugate B<Br>Antibody-Conjugate Dilution Buffer<Br>Standard<Br>Master Mix<Br>Ligase
• Quantity: 5 x 96 Tests
• Sample Volume: Serum, 2-5 μL; Supernatant, 2-5 μL; Plasma, 2-5 μL
• Target: Human
• Total Assay Time: 2 hr.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov