Learn More
Invitrogen™ pcDNA™4/HisMax A, B, & C Mammalian Expression Vectors
Designed to maximize protein expression in a variety of mammalian cells
Supplier: Invitrogen™ V86420
Includes: The pcDNA4/HisMax A, B and C vectors, 20μg each, are supercoiled and provided lyophilized. 20μg of pcDNA4/HisMax/lacZ control plasmid is also provided. The pcDNA4/HisMax TOPO TA Expression Kit contains two boxes. The TOPO TA box contains 200ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid. The box contains transformation reagents including 21 x 50μL aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid.
Description
- Contains QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with promoter alone
- In addition to SP163-enhanced expression, pcDNA4/HisMax includes cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with Anti-Xpress Antibody
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Transfection | |
Mammalian Expression Vector | |
The pcDNA™4/HisMax A, B, & C vectors, 20 μg each, are supercoiled and provided lyophilized. 20 μg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO™TA Expression Kit contains two boxes. The TOPO™ TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO™ vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid. |
|
His Tag (6x), Xpress Epitope Tag | |
pcDNA | |
Constitutive Expression |
CMV | |
Zeocin™ | |
EK (Enterokinase) Recognition Site | |
Restriction Enzyme/MCS | |
pcDNA™ |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.