Invitrogen™ pBAD/His Kit

Express the protein of interest with an N-terminal tag in a tightly regulated fashion

Supplier:  Invitrogen™ V43001

Catalog No. V43001

Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Description

• araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coli expression
• N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
• N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
• Enterokinase cleavage site for removing the N-terminal tag following purification
• Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Promoter: araBAD
• Selection Agent (Eukaryotic): None
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Protein Tag: His Tag (6x)
• Vector: pBAD

• Inducing Agent: Arabinose
• Product Type: Bacterial Expression Vector
• Content And Storage: The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
• Cleavage: EK (Enterokinase) Recognition Site
• Cloning Method: Restriction Enzyme/MCS

For Research Use Only. Not for use in diagnostic procedures.