Invitrogen™ pBAD/gIII Kit

Express recombinant proteins under the tight regulation of the araBAD promoter

Supplier:  Invitrogen™ V45001

Catalog No. V45001

Includes: T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Description

• To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
• Features araBAD promoter for tightly regulated expression
• Translation initiation signals for optimized E. coliexpression
• Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
• A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Promoter: araBAD
• Selection Agent (Eukaryotic): None
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cloning Method: Restriction Enzyme/MCS

• Inducing Agent: Arabinose
• Product Type: Bacterial Expression Vector
• Content And Storage: The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: c-Myc Epitope Tag
• Vector: pBAD

For Research Use Only. Not for use in diagnostic procedures.