Invitrogen™ Human Cot-1 DNA™

Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids

Supplier:  Invitrogen™ 15279011

Catalog No. 15-279-011

Includes: This DNA is supplied at 1mg/mL in 10mM Tris-HCl (pH 7.4), 1mM EDTA.

Description

Human Cot-1 DNA is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic clones to panels of somatic-cell hybrids for chromosome localization by Southern blotting.
About Cot-1 DNA
Human Cot-1 DNA is placental DNA that is predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members. The amount supplied per package is sufficient for 5–10 Southern or 500 in situ hybridizations.

Performance and quality testing
Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Human Cot-1 DNA 1:100 in 50mM NaOH and using the conversion factor 0.033μg/μL/A₂₆₀. Other methods used to determine concentration may yield varying results.

Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Product Type: DNA QC
• Reagent Type: Cot-1 DNA
• Species: Human
• For Use With (Application): Block Non-Specific Hybridization

• Content And Storage: This DNA is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA.
  Store at -20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Product Line: Cot-1 DNA™
• Quantity: 500 μg

For Research Use Only. Not for use in diagnostic procedures.