Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Supplier: Invitrogen™ K250020
Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.
Description
- Contains minimal MCS, so sequence more of insert and less of vector
- Patented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
- att sites for rapid recombination into a variety of Gateway destination vectors
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- Sequencing primer sites within 55 bp of insertion site
- Spectinomycin resistance gene for robust selection in E. coli
- EcoRI sites flanking the PCR product insertion site for easy excision of inserts
Cloning,Gateway Cloning,PCR Cloning
Specifications
TOPO™ with Gateway™ | |
One Shot™ | |
pCR |
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
TA Cloning Kit |
Provide Content Correction
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?
Provide Content Correction