Invitrogen™ Flp-In™ Complete System

Allows integration and expression of gene of interest in mammalian cells at specific genomic location

Supplier:  Invitrogen™ K601001

Catalog No. K601001

Includes: The Flp-In Complete System includes the Core Kit (Cat. No. K601002), 1g Zeocin antibiotic, and 1g hygromycin B. All vectors are supplied supercoiled and lyophilized.

Description

• Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
• Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site
• Major components include Flp-In. target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site
• Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter
• Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter
• Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In. host cell line, expresses CAT

Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Zeocin™, Hygromycin
• Protein Tag: Untagged
• Vector: pFRT
• For Use With (Application): Stable Cell Line Development, Targeted Integration

• Delivery Type: Transfection
• Product Type: Flp-In Complete System
• Content And Storage: The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.
• Cloning Method: Restriction Enzyme/MCS
• Product Line: Flp-In™, pcDNA™

For Research Use Only. Not for use in diagnostic procedures.