Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1

Dynabeads MyOne Streptavidin C1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin.

Supplier:  Invitrogen™ 65002

Catalog No. 65-002

Includes: Dynabeads™ are supplied in PBS, pH 7.4 with 0.01% Tween™-20 and 0.02% NaN₃ added as preservatives.

Description

Dynabeads MyOne Streptavidin C1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin. The product is intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands. Their small but uniform size presents a high surface area per mg of beads and a correspondingly high capacity for the target ligand, while the high iron content ensures an efficient magnetic pull. These features, along with a slow sedimentation rate during incubation contribute to a gentle and rapid magnetic separation. The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols.

Benefits of Dynabeads MyOne Streptavidin C1 beads include:
• Performance—high sensitivity and reproducibility secure consistent results
• Efficiency-—ast binding kinetics and high binding capacity
• Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System

Consistency and reproducibility
The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.

High binding capacity
The size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads MyOne Streptavidin C1 typically binds:
• >2,800 pmoles free biotin
• ∼20 µg biotinylated IgG 
• ∼400 pmol biotinylated peptides 
• ∼20 µg ds-DNA 
• ∼500 pmol ss-oligonucleotides

Applications 
Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications. Although the beads can be used to isolate any biotinylated targets, these hydrophilic beads are particularly well suited for the preparation of ssDNA, high-throughput nucleic acid clean-up protocols, and protein purifications prior to mass spectrometry.

Automation ready with the KingFisher Purification System
The 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.

Commercial supply 
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads MyOne Streptavidin C1 beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.

Order Info

Shipping Condition: Room Temperature

Specifications

• Binding Property: >2,500 pmoles free biotin/mg beads
• Concentration: 10 mg/mL
• Description: Recombinant streptavidin covalently coupled to Dynabeads
• For Use With (Application): Capture of Biotinylated Targets
• Product Type: Streptavidin Magnetic Beads
• For Use With (Equipment): KingFisher™ Sample Purification System, DynaMag™ magnets
• Isolation Technology: Magnetic Beads
• Preservative: 0.09% NaN₃
• Shipping Condition: Ambient Temperature
• Surface Functionality: Carboxylic Acid, Hydrophilic
• Diameter (Metric): 1 μm

• Certifications/Compliance: ISO9001 and ISO13485
• Content And Storage: Dynabeads MyOne Streptavidin C1 are supplied in PBS, pH 7.4, with 0.01% Tween-20 and 0.09% NaN₃. Store at 2–8°C.
  
  
• Color: Brown
• Material: Magnetic Polystyrene
• Format: Beads in suspension
• High-throughput Compatibility: Yes
• Ligand Type: Streptavidin
• Sample Type: Any Sample Type
• Size Distribution: CV <5%
• Volume (Metric): 10 mL
• Regulatory Status: For Research Use Only

Safety and Handling

ShelfLife : 36 months from date of manufacture

For Research Use Only. Not for use in diagnostic procedures.