Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1

Exceptional standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets

Supplier:  Invitrogen™ 65001

Catalog No. 65-001

Includes: Dynabeads™ are supplied in PBS, pH 7.4 with 0.01% Tween™-20 and 0.02% NaN₃ added as preservatives.

Description

Uniform and superparamagnetic beads are 1µm in diameter with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged, and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of results. The 1µm Dynabead MyOne have a large surface area, high capacity, efficient magnetic pull and a slow sedimentation rate during incubation. Tailor-made for use in automated protocols where high throughput is crucial. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability of results.

• ery high binding affinity of the streptavidin-biotin interaction (K_d=10^-15) is used in a vast number of applications
• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Well suited for nucleic acid applications with extreme demands
• Very low nonspecific binding of nucleotides and nucleic acids
• Low nonspecific binding of small and negatively charged proteins
• Their small but uniform size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• Low sedimention rate, yet a high iron content, ensuring rapid magnetic separation
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application
• Production follows a validated process in compliance with cGMP for medical devices

DNA and RNA Purification and Analysis, DNA Extraction, Immunoprecipitation, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis, RNAi, Epigenetics and Noncoding RNA Research, Sequence-Specific DNA or RNA Purification, Viral RNA/DNA Purification

Order Info

Shipping Condition: Room Temperature

Specifications

• Concentration: 10 mg/mL
• Sample Type: Any Sample Type
• Binding Property: >2,500 pmoles/mg beads
• High-throughput Compatibility: High-throughput Compatible
• Isoelectric Point: pH 5.2
• Size Distribution: CV < 5%
• Form: Beads in Suspension
• Ligand: Streptavidin
• Host Species: Recombinant
• Target Molecule: Biotin

• Quantity: 2 mL
• Content And Storage: Dynabeads™ are supplied in PBS, pH 7.4 with 0.01% Tween™-20 and 0.09% NaN₃. Store at 2-8°C
• Diameter (Metric) Bead: 1 μm
• Iron Content Ferrites: 26%
• Shipping Condition: Room Temperature
• Surface Functionality: Carboxylic Acid, Hydrophilic
• Product Line: Dynabeads™, MyOne™
• Purity or Quality Grade: Research Grade
• Product Type: Streptavidin Conjugate

For Research Use Only. Not for use in diagnostic procedures.