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Invitrogen™ Bluo-Gal
Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate.
Supplier: Invitrogen™ 15519028
Catalog No. 15-519-028
Description
Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. As with X-gal, Bluo-Gal produces blue (lac+) bacterial colonies and blue (lac+) viral plaques. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.
Bluo-Gal can also be used as a substrate with the streptavidin-β-galactosidase conjugate in both immunoblot and immunocytochemistry assays. In the presence of β-galactosidase, Bluo-Gal yields a blue precipitate, which is insoluble in alcohols and xylenes. Bluo-Gal is soluble in dimethylformamide.
Primary applications
• Blue-white screening (deeper blue as compared to X-gal)—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• Bluo-Gal overlay assay
Find the media format that fits your needs
• Ready-to-use media formulations are available, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301), or S.O.C recovery medium (Cat. No. 15544034).
• IPTG (Cat. No.15529019) is available for blue-white screening.
Order Info
Shipping Condition: Room Temperature
Specifications
| • Bluo-Gal, substrate for β-galactosidase (1 g) Store at –5°C to –30°C. |
|
| Colorimetric | |
| Blue/White Colony Screening, Immunoblotting, Immunocytochemical Assays | |
| 1 g |
| Bluo-Gal, Cell-Permeant | |
| Bottle | |
| Halogenated Indolyl-β-galactoside Substrate | |
| Approved for shipment at Room Temperature or on Wet Ice |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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