Invitrogen™ BLOCK-iT™ Adenoviral RNAi Expression System

Used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector

Supplier:  Invitrogen™ K494100

Catalog No. K494100

Includes: The pAd/BLOCK-iT™ RNAi Gateway™ Vector is provided supercoiled at a concentration of 150ng/μL and includes 10μg of positive control vector. The BLOCK-iT™ RNAi Adenoviral Expression System includes the BLOCK-iT™ U6 Entry Vector Kit, the pAd/BLOCK-iT™-DEST vector and control, the 293A Cell line, and LR Clonase™ enzyme mix.

Description

A novel cloning process places an approx. 50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector. After efficient recombination of the entry vector into the pAd/BLOCK-iT-DEST vector, followed by viral production and transduction, the shRNA driven by the U6 promoter can be transiently expressed in most dividing or non-dividing mammalian cell types. The shRNA generated avoids the hosts defense mechanism and will be effective at producing the RNAi gene knockdown response.

The pAd/BLOCK-iT-DEST vector offers:

• A promoterless region surrounded by attR sites for efficient recombination with the attL-flanked U6 Gateway entry vector containing the RNAi cassette or any attL-flanked promoter and gene sequence
• All of the required components for efficient adenoviral packaging and delivery of the shRNA of interest. With this vector, transient analysis of gene knockdown in both dividing and non-dividing mammalian cell types and animal models can be achieved.

Adenoviral shRNA, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Virus-Based RNAi

Specifications

• Product Type: RNAi Expression Vector Kit
• Cloning Method: Gateway™
• Delivery Type: Adenoviral
• Vector: pAd
• Product Line: BLOCK-iT™

• Content And Storage: The pAd/BLOCK-iT™ RNAi Gateway™ Vector is provided supercoiled at a concentration of 150 ng/μl and includes 10 μg of positive control vector. The BLOCK-iT™ RNAi Adenoviral Expression System includes the BLOCK-iT™ U6 Entry Vector Kit, the pAd/BLOCK-iT™-DEST vector and control, the 293A Cell line, and LR Clonase™ enzyme mix. Store vectors at -20°C. Store the cell line in liquid nitrogen. Store the LR Clonase™ mix at -80°C. Guaranteed stable for 6 months when properly stored.
• Constitutive or Inducible System: Constitutive
• RNAi Type: shRNA
• Promoter: U6
• Quantity: 20 Reactions

For Research Use Only. Not for use in diagnostic procedures.