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Invitrogen™ 3' RACE System for Rapid Amplification of cDNA Ends
Converts up to 1μg of total RNA preparation or 100ng of control RNA into cDNA in one reaction
Supplier: Invitrogen™ 18373019
Includes: 3' RACE System components are listed in Table 1.
Description
3' RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3' poly(A) end. The system is useful for the amplification of rare messages for which little sequence information is available, and for capturing the 3' end information of mRNA.
- Can be used with 5' RACE System to clone full-length sequences
- Uses advanced capabilities of SuperScriptII RT for greater first-strand cDNA yields
- Includes Control RNA and primers to monitor performance of system
- Provides detailed protocols in manual
Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction
Specifications
cDNA library | |
3´ RACE System components are listed in Table 1. Store the system at -20°C. Guaranteed stable for 6 months when properly stored. | |
cDNA Libraries & Library Construction | |
20 Reactions |
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For Research Use Only. Not for use in diagnostic procedures.