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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)

Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.

Supplier:  Invitrogen™ 88882388


Catalog No. 50-112-8915

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Contains Sodium azide and formaldehyde

Includes: Brefeldin A solution
IC Fixation Buffer
Permeabilization Buffer (10X) . Permeabilization Buffer is supplied as a 10X concentrate. 1X Permeabilization Buffer contains 0.1% saponin and 0.09% sodium azide.



  • Fixation buffer is used to cross-link, stabilize, and fix the cell membrane
  • Permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively


Fixation Reagent, Permeabilization Reagent


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