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Innovating Science™ AP Biology Investigation Kit No. 9: Restriction Enzyme Analysis

In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis.

Supplier:  Innovating Science™ IS3709

Catalog No. S07059

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  • Prepared agarose, 0.8%, 200mL TBE buffer, 5X, 500mL
  • DNA Stain, 20X, 60mL DNA Samples, 100μL each:
  • DNA Marker (Lambda DNA HindIII digest)
  • Sample #1 (Lambda DNA)
  • Sample #2 (Lambda DNA BstEII digest)
  • Sample #3 (Lambda DNA EcoRI digest)
  • Sample #4 (Lambda DNA BstEII digest)

Requires :
  • Agarose electrophoresis chamber
  • Microwave or hot water bath
  • Electrophoresis power supply
  • Distilled or deionized water
  • Micropipettes w/micropipette tips, 10μL
  • Gel staining trays or similar (large enough container to accommodate flat agarose gels)



Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples. Meets AP Science Practices 3 and 6, and Big Idea 3. Materials provided for eight lab groups.



Meets AP science practices 3 and 6 and big idea 3
13 to 20
Prepared agarose, 0.8%, 200mL;  TBE buffer, 5X, 500mL; DNA Stain, 20X, 60mL; DNA Samples, 100μL each: DNA Marker (Lambda DNA HindIII digest), Sample 1 (Lambda DNA), Sample 2 (Lambda DNA BstEII digest), Sample 3 (Lambda DNA EcoRI digest), Sample 4 (Lambda DNA BstEII digest)
9.75 x 6.25 x 4 in.
AP Biology Investigation Kit #9: Biotechnology: Restriction Enzyme Analysis of DNA
7 to 14
Students will understand the action and specificity of restriction enzymes
8 Lab Groups or 32 Students


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