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Vector Laboratories ImmPRESS-HRP Anti-Rat IgG, Mouse-Adsorbed (Peroxidase) Polymer Detection Kit, Made In Goat 15 ml
Supplier: Vector Laboratories MP744415
This ImmPRESS-HRP Anti-Rat IgG Mouse-Adsorbed (Peroxidase) Polymer Detection Kit is made in goat and targets rat primary antibodies. Mouse adsorption minimizes interference from endogenous mouse IgG when the system is used on mouse tissue. The ImmPRESS reporter enzyme staining system uses novel conjugation chemistry to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. A combination of highly active enzyme (peroxidase or alkaline phosphatase) and affinity-purified secondary antibodies results in crisp, strong staining. The IHC immunostaining protocol is simple: Following a blocking step with 2.5% goat serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added and incubated for 30 minutes. Sections are again rinsed and the slides are developed with a peroxidase or alkaline phosphatase substrate. The ImmPRESS Reagent is ready-to-use and stains 75-150 sections.
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