Cytiva Tissue and Cells genomicPrep Mini Spin Kit

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures

Supplier:  Cytiva 28904276

Catalog No. 45-001-503

Includes: Spin columns prepacked with a novel silica-membrane; Lyophilized proteinase K powder; Lysis solutions, wash and elution buffers; 2mL Microcentrifuge collection tubes; Full protocol booklet with a detachable, quick reference protocol card

Description

To minimize the incidence of DNA shearing during extraction, the illustra tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells. The kit can be used to extract DNA from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A_260/280 readings of about 1.8.

• Capable of purifying up to 1.5μg/mg of tissue from an input tissue sample range of 5 to 50mg, and up to 40μg of genomic DNA per 5 million cells depending on cell type
• Total DNA extraction time reduced significantly to 90 minutes (including tissue disruption)
• Produces high-quality genomic DNA with high molecular weight and less degradation
• Validated in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

Specifications

• Description: illustra tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from a variety of animal tissues and mammalian cell cultures in just 90 min for tissues and 45 min for ce
• Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
• Product Type: illustra tissue and cells genomicPrep Mini Spin Kit
• Sample Volume: 5-50 mg

• For Use With (Application): Used for several downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR
• No. of Reactions: 250 Preps
• Purity: 1.75
• Test Time: 90 min[Animal Tissue], 45 min[Cultured cells]