Cytiva RNAspin Mini Isolation Kit

Extract high-quality RNA from a wide range of samples rapidly. Cytiva Life Sciences™illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast.

Includes: Protocol booklet with detachable, quick reference card, necessary components for specified number of preps, including prefilters and DNase I

Catalog Number	Mfr. No.	No. of Reactions
45-001-161	Cytiva 25050070	20 Preps
45-001-163	Cytiva 25050072	250 Preps
45-001-162	Cytiva 25050071	50 Preps

Description

• Recovers total RNA due to removal of genomic DNA through on-column DNase I treatment
• Can be used on cultured cells, tissue, yeast and hard-to-lyse bacterial strains
• Prefilters reduce lysate viscosity for maximum yield and purity
• Results can be obtained with small amounts of sample
• Binding capacity: 100μg
• Sample size: 30mg (animal tissue), up to 5 × 10⁶ cells (cell culture)
• Elution volume: 40 to 120μL
• RNA Purity (A260/A280): 1.8 to 2.2
• Total time of preparation: <30 min/6 preps

Quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments

Specifications

• Description: illustra RNAspin Mini RNA Isolation Kit is designed for rapid extraction of high-quality RNA from a wide range of sample types.
• Includes: Protocol booklet with detachable, quick-reference guide and necessary components, including prefilters and DNase I
• RNase: 1.8-2.2
• Test Time: < 30 min/6 preps

• For Use With (Application): Used for the isolation of high-quality total RNA from a wide range of source material including hard-to-lyse bacterial strains. Purified RNA is suitable for use in sensitive downstream applications such as quantitative reverse transcriptase PCR (qRT-PCR),
• Product Type: illustra RNAspin Mini Kit
• Sample Volume: <30 mg
• Yield: Up to 100μg