Cytiva plasmidPrep Midi Flow Kit

For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification

Supplier:  Cytiva 28904267

Catalog No. 45-001-498

Includes: Fast-flow plasmid 250 columns; Resuspension, lysis, neutralization, wash, elution, and TE buffers; Full protocol booklet with a detachable, quick reference protocol card

Description

The kit consists of a high-performance, anion-exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.

• High column capacity allows for a proportional yield for input sample volume of 25 to 50mL (for high copy number plasmid) and up to 500mL for low copy number plasmid
• Provides plasmid DNA with excellent purity and low endotoxin levels
• Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns

Specifications

• Description: illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.
• Includes: Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card
• Product Type: illustra plasmidPrep Midi Flow Kit
• Sample Volume: 150 mL Culture

• For Use With (Application): Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification
• No. of Reactions: 25 Preps
• Purity: 1.8
• Test Time: Approx.215 min