Learn More
Invitrogen™ IL-6 Human Matched Antibody Pair
Matched Antibody Pair
Supplier: Invitrogen™ BMS2132MST
Includes: 1 vial (2.75mL) monoclonal Coating Antibody to human IL-6 (100μg/mL)
1 vial (55μL) Biotin-Conjugate anti-human IL-6 monoclonal antibody
1 vial (22μL) Streptavidin-HRP
1 vial human IL-6 Standard protein lyophilized, 2ng /mL upon reconstitution
Description
The Human IL-6 Matched Antibody Pair Kit comes with pre-matched antibody pairs,standards,and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury,and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts,activated T cells,activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells,T cells,B cells,and hepatocytes. In addition,IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation,IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor,alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.Specifications
IL-6 | |
Monoclonal | |
P05231 | |
IL6 | |
RUO | |
3569 | |
-20°C |
ELISA | |
Biotin | |
Interferon-beta 2, B-cell stimulatory factor 2,HGF | |
10 x 96 Tests | |
Primary | |
Human | |
ELISA Kit |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
For Research Use Only.