Invitrogen™ IL-2 Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ BMS2212TEN

Catalog No. BMS2212TEN

Description

The Human Programmed T cell Death Ligand 1 (Hu PD-L1) ELISA quantitates Hu PD-L1 in human serum,plasma,cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-L1. Principle of the method The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

PD-L1 (Programmed death receptor ligand 1, B7-H1, CD274) is a a 40 kDa transmembrane protein and B7 family member. PD-L1 has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production, and PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion. Together with B7.1, PD-L1 can inactivate T cells and this relationship is a focal point of cancer therapeutic treatment. At least two isoforms of PDL-1 are known to exist.

Specifications

• Accession Number: Q9NZQ7
• Assay Sensitivity: 0.6 pg/ml
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 29126
• Interassay CV: 3.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Cell Lysate,50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 4 hr.

• Assay Range: 4.69-300.0 pg/ml
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD274
• Intraassay CV: 2.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.