Thermo Scientific™ Hypersil™ PREP HS C8 HPLC Columns

Use these preparative columns for scale-up purification with high phase loading. They are suitable for all USP-L7 applications.

Catalog Number	Mfr. No.	Particle Size	Diameter (Metric)	Length (Metric)	Column Format
03-252-999	Thermo Scientific™ 41103052130	3 μm	2 mm	50 mm	Preparative Column, Standard
03-253-007	Thermo Scientific™ 41103054630	3 μm	4.6 mm	50 mm	Preparative Column, Standard
03-253-000	Thermo Scientific™ 41103102130	3 μm	2 mm	100 mm	Preparative Column, Standard
03-253-008	Thermo Scientific™ 41103104630	3 μm	4.6 mm	100 mm	Preparative Column, Standard
03-253-001	Thermo Scientific™ 41103152130	3 μm	2 mm	150 mm	Preparative Column, Standard
03-253-009	Thermo Scientific™ 41103154630	3 μm	4.6 mm	150 mm	Preparative Column, Standard
03-253-002	Thermo Scientific™ 41103252130	3 μm	2 mm	250 mm	Preparative Column, Standard
03-253-010	Thermo Scientific™ 41103254630	3 μm	4.6 mm	250 mm	Preparative Column, Standard
03-253-003	Thermo Scientific™ 41105052130	5 μm	2 mm	50 mm	Preparative Column, Standard
03-253-011	Thermo Scientific™ 41105054630	5 μm	4.6 mm	50 mm	Preparative Column, Standard
03-253-015	Thermo Scientific™ 41105059070A	5 μm	10 mm	50 mm	Preparative Column, Standard
03-253-016	Thermo Scientific™ 41105059270A	5 μm	20 mm	50 mm	Preparative Column, Standard
03-253-044	Thermo Scientific™ 41105059270XL	5 μm	25 mm	50 mm	Preparative Column, XtendedLife
03-253-004	Thermo Scientific™ 41105102130	5 μm	2 mm	100 mm	Preparative Column, Standard
03-253-012	Thermo Scientific™ 41105104630	5 μm	4.6 mm	100 mm	Preparative Column, Standard
03-253-017	Thermo Scientific™ 41105109070A	5 μm	10 mm	100 mm	Preparative Column, Standard
03-253-018	Thermo Scientific™ 41105109270A	5 μm	20 mm	100 mm	Preparative Column, Standard
03-253-045	Thermo Scientific™ 41105109270XL	5 μm	25 mm	100 mm	Preparative Column, XtendedLife
03-253-005	Thermo Scientific™ 41105152130	5 μm	2 mm	150 mm	Preparative Column, Standard
03-253-013	Thermo Scientific™ 41105154630	5 μm	4.6 mm	150 mm	Preparative Column, Standard
03-253-019	Thermo Scientific™ 41105159070A	5 μm	10 mm	150 mm	Preparative Column, Standard
03-253-020	Thermo Scientific™ 41105159270A	5 μm	20 mm	150 mm	Preparative Column, Standard
03-253-046	Thermo Scientific™ 41105159270XL	5 μm	25 mm	150 mm	Preparative Column, XtendedLife
03-253-021	Thermo Scientific™ 41105159370A	5 μm	30 mm	150 mm	Preparative Column, Standard
03-253-047	Thermo Scientific™ 41105159370XL	5 μm	30 mm	150 mm	Preparative Column, XtendedLife
03-253-022	Thermo Scientific™ 41105159570A	5 μm	50 mm	150 mm	Preparative Column, Standard
03-253-048	Thermo Scientific™ 41105159570XL	5 μm	50 mm	150 mm	Preparative Column, XtendedLife
03-253-006	Thermo Scientific™ 41105252130	5 μm	2 mm	250 mm	Preparative Column, Standard
03-253-014	Thermo Scientific™ 41105254630	5 μm	4.6 mm	250 mm	Preparative Column, Standard
03-253-023	Thermo Scientific™ 41105259070A	5 μm	10 mm	250 mm	Preparative Column, Standard
03-253-024	Thermo Scientific™ 41105259270A	5 μm	20 mm	250 mm	Preparative Column, Standard
03-253-049	Thermo Scientific™ 41105259270XL	5 μm	25 mm	250 mm	Preparative Column, XtendedLife
03-253-025	Thermo Scientific™ 41105259370A	5 μm	30 mm	250 mm	Preparative Column, Standard
03-253-050	Thermo Scientific™ 41105259370XL	5 μm	30 mm	250 mm	Preparative Column, XtendedLife
03-253-026	Thermo Scientific™ 41105259570A	5 μm	50 mm	250 mm	Preparative Column, Standard
03-253-051	Thermo Scientific™ 41105259570XL	5 μm	50 mm	250 mm	Preparative Column, XtendedLife
03-253-039	Thermo Scientific™ 41110018823A	10 μm	10 mm	8 mm	Guard Column
03-253-040	Thermo Scientific™ 41110019023A	10 μm	10 mm	20 mm	Guard Column
03-253-042	Thermo Scientific™ 41110019323A	10 μm	10 mm	30 mm	Guard 1/16 in.
03-253-060	Thermo Scientific™ 41110019323B	10 μm	10 mm	30 mm	Guard 1/8 in.
03-253-043	Thermo Scientific™ 41110029323A	10 μm	20 mm	30 mm	Guard 1/16 in.
03-253-061	Thermo Scientific™ 41110029323B	10 μm	20 mm	30 mm	Guard 1/8 in.
03-253-027	Thermo Scientific™ 41110059070A	10 μm	10 mm	50 mm	Preparative Column, Standard
03-253-028	Thermo Scientific™ 41110059270A	10 μm	20 mm	50 mm	Preparative Column, Standard
03-253-052	Thermo Scientific™ 41110059270XL	10 μm	25 mm	50 mm	Preparative Column, XtendedLife
03-253-029	Thermo Scientific™ 41110109070A	10 μm	10 mm	100 mm	Preparative Column, Standard
03-253-030	Thermo Scientific™ 41110109270A	10 μm	20 mm	100 mm	Preparative Column, Standard
03-253-053	Thermo Scientific™ 41110109270XL	10 μm	25 mm	100 mm	Preparative Column, XtendedLife
03-253-031	Thermo Scientific™ 41110159070A	10 μm	10 mm	150 mm	Preparative Column, Standard
03-253-032	Thermo Scientific™ 41110159270A	10 μm	20 mm	150 mm	Preparative Column, Standard
03-253-054	Thermo Scientific™ 41110159270XL	10 μm	25 mm	150 mm	Preparative Column, XtendedLife
03-253-033	Thermo Scientific™ 41110159370A	10 μm	30 mm	150 mm	Preparative Column, Standard
03-253-055	Thermo Scientific™ 41110159370XL	10 μm	30 mm	150 mm	Preparative Column, XtendedLife
03-253-034	Thermo Scientific™ 41110159570A	10 μm	50 mm	150 mm	Preparative Column, Standard
03-253-056	Thermo Scientific™ 41110159570XL	10 μm	50 mm	150 mm	Preparative Column, XtendedLife
03-253-035	Thermo Scientific™ 41110259070A	10 μm	10 mm	250 mm	Preparative Column, Standard
03-253-036	Thermo Scientific™ 41110259270A	10 μm	20 mm	250 mm	Preparative Column, Standard
03-253-057	Thermo Scientific™ 41110259270XL	10 μm	25 mm	250 mm	Preparative Column, XtendedLife
03-253-037	Thermo Scientific™ 41110259370A	10 μm	30 mm	250 mm	Preparative Column, Standard
03-253-058	Thermo Scientific™ 41110259370XL	10 μm	30 mm	250 mm	Preparative Column, XtendedLife
03-253-038	Thermo Scientific™ 41110259570A	10 μm	50 mm	250 mm	Preparative Column, Standard
03-253-059	Thermo Scientific™ 41110259570XL	10 μm	50 mm	250 mm	Preparative Column, XtendedLife

Description

Get maximum loading for your scale up from analytical to a prep LC method with Thermo Scientific™ Hypersil™ PREP HS C8 reversed phase preparative HPLC column. With similar selectivity to C18 columns and predictable elution order, C8 columns are ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. By providing high surface area particles with a 100Å pore size, and a variety of dimensions including an option for XtendedLife hardware, which improves column lifetime by maintaining a uniform packed bed.

Similar selectivity, less retention than C18 columns

• Successful retention and separation of moderately hydrophobic analytes.
• Ideal for fatty acids, which are too hydrophobic for analysis using C18 columns.
• C8 (USP L7) selectivity - high surface area particle with 100Å pore size.
• pH stable from 2 to 8.
• Maximum temperature of 60°C.

Faster separations

• Higher analytical throughput compared to C18 alkyl chain chemistry.
• Quicker compound elution due to reduced hydrophobic interactions.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

Specifications

• pH: 2 to 8
• Surface Area: 350 m²/g
• USP Type: L7
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Column Type: Reversed Phase
• Phase: Reversed Phase

• Pore Size: 100 Å
• Temperature: 60°C
• Stationary Phase: C8
• Carbon Load: 7%
• Endcapped: Yes